• Runs in Windows (XP, Vista, 7, 8, and 10) and Mac (OS X v10.5 and above)
• Highlights restriction sites in the editing window
• Accurately reflects Dam/Dcm blocking of enzyme sites
• Highlights text using pre-defined and custom feature libraries
• Shows translation, Tm, %GC, ORF of selected DNA in real-time
• Reads DNA Strider, Fasta, Genbank and EMBL files
• Saves files as DNA Strider-compatible or Genbank file format
• Highlights and draws graphic maps using feature annotations from genbank and embl files
• Directly BLASTs selected sequence at NCBI or wormbase

• Text map shows DNA sequence, translation, and features as text-based graphics

• Creates graphic restriction maps- linear or circular with features indicated
• Connects graphic and text features with hyperlink double click
• Saves graphics as encapsulated postscript or scalable vector graphics
• Copy and save graphics as Windows metafiles (MS Windows only)

• Virtual restriction digest
• Draws pre-defined and user-defined DNA ladders
• Connects bands to text by double-click

• Reads ABI sequencing trace files
• Sequences in ABI traces can be aligned directly to a reference sequence, with the alignment hyperlinked back to te trace.

• Selects sites matching multiple criteria (union/intersection- cut frequency, site type) in all open windows
• Selects sites that cut more often in one sequence than another (for snip-SNP detection or diagnostic digests)
• Has user defined enzyme grouping to distiguish eg. enzymes currently in stock.
• Allows users to define new enzymes by name and recognition site
• Imports DNA Strider format files (simple enzyme, site lists) available from REBASE

Other Features:

• Most analysis windows are hyperlinked to their corresponding sequences, including:
  • Graphic Maps
  • Text maps
  • Virtual Digests
  • Alignments (including ABI sequences)
  • Silent Sites
  • Translation
  • Primer Find
• Uses custom feature definition libraries, which allow:
  • Quick annotation of sequence
  • Quick searching and highlighting of all available primers that you (or others) have that hybridize to a sequence
  • Sequence to be annotated and visualized in multiple ways quickly and efficiently
  • Graphic maps that show primer binding sites and all interesting sequence features
• Translates sequences with optional DNA alignment
• Finds potential primers matching user criteria (length, Tm, %GC, self/other complementarity)
• Aligns two DNA sequences (or any combination of sequence and ABI trace), with the alignment hyperlinked to the original sequence
• Finds translationally silent restriction sites
• Draws graphic ORF maps